Thursday, January 19, 2017

Gel Electrophoresis Lab

When we analyzed the gel, there were  only a little difference from our reference dye and candy extracted dye. It was mainly color wise, but even so, it was by a couple of shades. The dyes stayed together for the most part, however the yellow managed to spread and cross the red dye. I think the dye that resembles the dyes we used would be the Fast Green FCF since the dyes we had migrated up and down and seemed to spread out from the side.

The main enticement for the sale of food items is how they look. If a hunk of meat looks rotten or grey, people simply wont buy it. In the sale of dog food, it doesn't matter to the dog how it looks; they are color blind, however, for people, the look of the food in their house or their dogs diet is incredibly important and so dog food companies invest in making the food more appetizing not for the dog, but for the human instead. Even the food that human eat, if mac and cheese is defined by the orange/ yellow cheese even if it is chemically based. 

The two things that control the migration of DNA is the electricity and the buffers acting on the gel. The electricity is the thing that acts on the DNA and causes it to move up the gel floor. The DNA groups by size of the dye and groups by their size automatically after adding electricity. I believe DNA with molecular weights in Daltons move and separate by size and weight. The 600s stay with the 600s and the 1000s with the 1000s and so forth.


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