PGLO Lab Analysis

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB none none none + pGLO LB/amp 37 grayish yellow white + pGLO LB/amp/ara 28 Greyish yellow Fluorescent yellow/ green

2.	What two new traits do your transformed bacteria have?
	They clump together in colonies and they have a fuzzy border around them and some have more lots of clear colonies but some have a mushy colony.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	It would be impossible to count exactly how much bacteria there is because live bacteria is constantly growing and expanding but if we moved one colony then I approximate that there was 100 plus live bacteria samples transferred.
4.	What is the role of arabinose in the plates?
	I believe the arabinose acts as a culture for the bacteria to help improve growth and promote the increase in bacteria sample.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	GFP can be used in military science as a glow in the dark mechanism for underwater exploration. It can be used to make glow sticks and those collars for dogs and people to keep them safe in the dark. It’s a hard question because GFP by itself is just a protein sample from a jellyfish, but maybe it can be used to insert in animals or people to make them glow in the dark.
6.	Give an example of another application of genetic engineering. Genetic engineering is the manipulation of genes and an example of that would be genetically modified food. Food that has had been altered and manipulated for maximum harvest counts as genetic engineering and is a common form of biotechnology.